Publications list with abstract anoxic tank

Abstract:

Conidiation is an asexual sporulation pathway that is a response to adverse conditions and is the main mode of dispersal utilized by filamentous fungal pathogens for reestablishment in a more favorable environment. Heterotrimeric G proteins (consisting of α, β, and γ subunits) have been shown to regulate conidiation in diverse fungi. Previous work has demonstrated that all three of the Gα subunits in the filamentous fungus neurospora crassa affect the accumulation of mass on poor carbon sources and that loss of gna-3 leads to the most dramatic effects on conidiation. In this study, we used 1H nuclear magnetic resonance (NMR) to profile the metabolome of N.


Crassa in extracts isolated from vegetative hyphae and conidia from cultures grown under conditions of high or low sucrose.Anoxic tank we compared wild-type and Δgna-3 strains to determine whether lack of gna-3 causes a significant difference in the global metabolite profile. The results demonstrate that the global metabolome of wild-type hyphae is influenced by carbon availability. The metabolome of the Δgna-3 strain cultured on both high and low sucrose is similar to that of the wild type grown on high sucrose, suggesting an overall defect in nutrient sensing in the mutant. However, analysis of individual metabolites revealed differences in wild-type and Δgna-3 strains cultured under conditions of low and high sucrose.

2.

Kayla A. Kaiser, gregory A. Barding, cynthia K. Larive. (2009). Metabolic profiling of plants by 1H-NMR: A comparison of metabolite extraction strategies using rosette leaves of the model plant arabidopsis thaliana.Anoxic tank magnetic resonance in chemistry 47: S147-S156.

Abstract

Article

Abstract:

Metabolite analysis is recognized as an important facet of systems biology, however complete metabolome characterization has not been realized due to challenges in sample preparation, inherent instrumental limitations and the labor intensive task of data interpretation. This work aims to compare several commonly used metabolite extraction strategies for their effect on the 1H nuclear magnetic resonance (NMR) metabolic profile of extracts of the model plant arabidopsis thaliana. Extractions were carried out on aliquots from a pool of homogenized plant tissue using CD3CN/D2O, buffered D2O, perchloric acid in D2O, CD3OD/D2O and CD3OD/D2O/cdcl3 as the extraction solvents.Anoxic tank the effects of lyophilization as a sample pretreatment, solvent evaporation and extract fractionation for removal of interfering species were studied. Representative spectra are presented for qualitative interpretation. Analytical reproducibility was evaluated by principal components analysis. Perchloric acid facilitated acid-catalyzed cleavage of sucrose, further complicating biological interpretation of the resulting metabolite profile. The solvent system CD3OD/D2O/cdcl3 gave the least reproducible results in our hands. D2O extracts suffered from poor stability probably due to contamination by soluble enzymes, which were not denatured in this solvent. CD3CN/D2O extracts showed greater stability than D2O alone, but problems were encountered due to degradation of 1H NMR spectral resolution during lengthy acquisitions due to partial phase separation.Anoxic tank in addition, this solvent system produced spectra with significant contamination by lipids that obscured spectral regions containing the resonances of the aliphatic amino acids. These problems were solved by speedvacuuming the CD3CN/D2O extract and reconstituting in D2O solution.

3.

Cristina branco-price, kayla A. Kaiser, charles J. H. Jang, cynthia K. Larive, julia bailey-serres. (2008). Selective mrna translation coordinates energetic and metabolic adjustments to hypoxia and reoxygenation in arabidopsis thaliana. The plant journal 56: 743–755.

Abstract

Article

Abstract:

Cellular oxygen deprivation (hypoxia/anoxia) requires an acclimation response that enables survival during an energy crisis. To gain new insights into the processes that facilitate the endurance of transient oxygen deprivation, the dynamics of the mrna translation state and metabolites were quantitatively monitored in arabidopsis thaliana seedlings exposed to a short (2 h) or prolonged (9 h) period of oxygen and carbon dioxide deprivation and following 1 h of re-aeration.Anoxic tank hypoxia stress and reoxygenation promoted adjustments in the levels of polyribosomes (polysomes) that were highly coordinated with cellular ATP content. A quantitative comparison of steady-state and polysomal mrna populations revealed that over half of the cellular mrnas were restricted from polysome complexes during the stress, with little or no change in abundance. This selective repression of translation was rapidly reversed upon reoxygenation. Comparison of the adjustment in gene transcripts and metabolites demonstrated that profiling of polysomal mrnas strongly augments the prediction of cellular processes that are altered during cellular oxygen deprivation. The selective translation of a subset of mrnas promotes the conservation of ATP and facilitates the transition to anaerobic metabolism during low-oxygen stress.Anoxic tank

Close this window