Fetal bovine serum suppresses apoptosis in the small intestine after total ischemia and reperfusion in mice – journal of pediatric surgery anoxic brain injury prognosis

Fig 1

The number of the apoptotic cells of the small intestine after total I/R in the FBS- and saline-treated mice. The mice were pretreated with either normal saline (saline, black bar) or fetal bovine serum (FBS, gray bar) for 3 days concluding with total ischemia of the small intestine (6 mice for each time-point). The mice in the sham-operated group (sham, white bar) were killed at the same time-points postsurgery (3 mice for each time-point). Sections of the small intestine at various time-points after ischemia or reperfusion (before and after 1 hour of ischemia, and at 1, 6, and 24 hours after reperfusion (0h, i1h, r1h, r6h, and r24h, respectively) were TUNEL stained.


The number of cells positively stained by TUNEL staining was counted under microscope (×200) and the apoptotic cell number in each section determined by averaging the cell numbers from 5 independent fields.Anoxic brain injury prognosis apoptotic cell number is presented as mean ± SD. The difference of the number of the apoptotic cells was compared among various groups using student’s t test for statistical analysis. Results were considered statistically significant where P .05. ∗indicates that the apoptotic cell number at that time-point was statistically different from the 0h of the same group. + indicates that the apoptotic cell number of the saline- or FBS-treated group at that time-point was statistically different from the sham-operated group at the same time point. # indicates that the apoptotic cell number of the FBS-treated group at that time-point was statistically lower than the saline-treated group at the same time-point.Anoxic brain injury prognosis

Fig 3

The activity of caspase-3 in the mouse small intestine after I/R. Caspase-3 activity in the mouse small intestine was analyzed after I/R using caspase activity assay: before and after 1 hour of ischemia and at 1, 6, and 24 hours after reperfusion (0h, i1h, r1h, r6h, and r24h, respectively). The mice were pretreated with either normal saline (saline, black bar) or fetal bovine serum (FBS, gray bar) for 3 days concluding with total ischemia of the small intestine (6 mice for each time-point). The mice in the sham-operated group (sham, white bar) were killed at the same time-points postsurgery (3 mice for each time-point). Results are expressed as the change in fluorescence (in arbitrary fluorescence unit; mean ± SD).Anoxic brain injury prognosis P .05 represents a significant difference. ∗ indicates that the caspase-3 activity at that time-point was statistically different from the 0h of the same group. + indicates that the caspase-3 activity of the saline- or FBS-treated group at that time-point was statistically different from the sham-operated group at the same time-point. # indicates that the caspase-3 activity of the FBS-treated group at that time-point was statistically lower than the saline-treated group at the same time-point.

Fig 1

The number of the apoptotic cells of the small intestine after total I/R in the FBS- and saline-treated mice. The mice were pretreated with either normal saline (saline, black bar) or fetal bovine serum (FBS, gray bar) for 3 days concluding with total ischemia of the small intestine (6 mice for each time-point).Anoxic brain injury prognosis the mice in the sham-operated group (sham, white bar) were killed at the same time-points postsurgery (3 mice for each time-point). Sections of the small intestine at various time-points after ischemia or reperfusion (before and after 1 hour of ischemia, and at 1, 6, and 24 hours after reperfusion (0h, i1h, r1h, r6h, and r24h, respectively) were TUNEL stained. The number of cells positively stained by TUNEL staining was counted under microscope (×200) and the apoptotic cell number in each section determined by averaging the cell numbers from 5 independent fields. Apoptotic cell number is presented as mean ± SD. The difference of the number of the apoptotic cells was compared among various groups using student’s t test for statistical analysis.Anoxic brain injury prognosis results were considered statistically significant where P .05. ∗indicates that the apoptotic cell number at that time-point was statistically different from the 0h of the same group. + indicates that the apoptotic cell number of the saline- or FBS-treated group at that time-point was statistically different from the sham-operated group at the same time point. # indicates that the apoptotic cell number of the FBS-treated group at that time-point was statistically lower than the saline-treated group at the same time-point.

Fig 3

The activity of caspase-3 in the mouse small intestine after I/R. Caspase-3 activity in the mouse small intestine was analyzed after I/R using caspase activity assay: before and after 1 hour of ischemia and at 1, 6, and 24 hours after reperfusion (0h, i1h, r1h, r6h, and r24h, respectively).Anoxic brain injury prognosis the mice were pretreated with either normal saline (saline, black bar) or fetal bovine serum (FBS, gray bar) for 3 days concluding with total ischemia of the small intestine (6 mice for each time-point). The mice in the sham-operated group (sham, white bar) were killed at the same time-points postsurgery (3 mice for each time-point). Results are expressed as the change in fluorescence (in arbitrary fluorescence unit; mean ± SD). P .05 represents a significant difference. ∗ indicates that the caspase-3 activity at that time-point was statistically different from the 0h of the same group. + indicates that the caspase-3 activity of the saline- or FBS-treated group at that time-point was statistically different from the sham-operated group at the same time-point. # indicates that the caspase-3 activity of the FBS-treated group at that time-point was statistically lower than the saline-treated group at the same time-point.Anoxic brain injury prognosis